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The specimens examined based on macro and micro-morphological characteristics.įor the isolation of causal agents, the diseased leaves and ears were cut into segments of 4–8 cm, surface-sterilized in 70% ethanol for 1 min, rinsed in sterile water and placed in glass slides with tape and kept under high humidity conditions until the pycnidia produced cirri containing pycnidiospores. Fresh samples were photographed before collection to save distinctive characters using camera EOS 1300D. Sampling and morphological characterizationĭuring surveys from 2016 to 2017, symptomatic leaves and ears were collected from different poaceous species in the field from three provinces of Iran including Fars, Khuzestan and Kohgiluyeh and Boyerahmad, and taken to the laboratory. Species identification was done according to the morphology of anamorph and molecular confirmation using ITS and LSU sequences. Therefore, this study was conducted to determine otherspecies of Parastagonospora in association with poaceous plants in Iran. Frank) (Pat5) have been reported based on molecular and morphological characteristics on poaceous plants in Iran (Ghaderi et al. So far, three species of the genus Parastagonosporaincluding P. 2007) and ß-xylosidase gene (McDonald et al. 2006), histidine synthase gene (Wang et al.

2005), RNA polymerase II (Arkadiusz et al. In the last two decades, the genus Parastagonospora was analyzed using different genes including mating-type loci (Bennett et al. Because of the overlapping observed in the morphological systematic studies within the genus, molecular techniques based on multilocus DNA sequencing analysis have enabled researchers to solve these problems (Quaedvlieg et al. Today, there are many deficiencies and problems of morphological taxonomy that have been solved using molecular techniques. Therefore, the name Parastagonospora has been established as the valid name instead of Phaeosphaeria(I.

LSU ENDNOTE SOFTWARE CODE

Since January 2013, following new rules for naming of pleomorphic fungi outlined in the International Code of Nomenclature for Algae, Fungi and Plants (ICN) as the pleomorphic fungus may have only one name (Wingfield et al. (2013) introduced and used a new genus Parastagonospora (Quaedvlieg, Verkley & Crous) instead of Stagonospora genus. The conidia of Stagonospora species were more than 10 times smaller than those of Septoria (Sprague, 1950). Based on conidial morphology, Septoria and Stagonospora (Sacc.) were grouped as two different genera and Stagonospora was accepted as the correct description in place of Septoria. Parastagonospora was first defined as Septoria (Sacc) (Weber 1922). Some species of the genus Parastagonospora are responsible for significant crop losses in wheat, barley and rye worldwide (Quaedvlieg et al. Parastagonospora (Berk.) Quaedvlieg, Verkley & Crous belonged to the class Dothideomycetes, order Pleosporales and family Phaeosphaeriaceae (Quaedvlieg et al.

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